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Amount 1AFigure 1Results of Stream Cytometry and Sequencing in the Index Patient. Shows the total results on flow cytometry. Whole-exome sequencing of purified CD8+ leukemic cells and CD4+ control cells from the index patient produced approximately 61.1 million and 60.2 million reads, respectively, that could be mapped to the reference genome . The paired-end read length was 82 nucleotides. After somatic mutation contacting and validation by capillary sequencing, the genes for transmission transducer and activator of transcription 3 , macrophage scavenger receptor 1 , and homeobox C9 were found to harbor somatic heterozygous mutations particular to the CD8+ cell population. The mutations in MSR1 and STAT3 presented an amino acid substitution in the encoded proteins, whereas the mutation in HOXC9 was a deletion of an amino acid .