Unlike the earlier edition of the assay, this clinical-grade assay could be transitioned seamlessly from translational analysis into clinical diagnostics. During the last several decades, FISH and IHC technologies have already been developed to detect proteins and DNA in situ, respectively. But reliable recognition of RNA in situ remains difficult, in routine medical specimens especially, stated Dr. Yuling Luo, Founder, CEO and President of ACD.Variants were annotated based on the effect on the proteins, novelty, conservation, and cells expression with the use of an automated pipeline for genome annotation.19 A novel variant was determined in DYRK1B, the gene encoding dual-specificity tyrosine-phosphorylation-regulated kinase 1B. In this variant, cysteine is definitely substituted for arginine at position 102 in DYRK1B . Functional Analysis of DYRK1B R102C We examined the consequences of expression of nonmutated DYRK1B and the novel variant DYRK1B R102C, along with the effects of DYRK1B knockdown using short hairpin RNA , on adipogenic differentiation in everlasting 3T3-L1 preadipocyte cell lines. We also examined the consequences of DYRK1B variants on the expression of glucose-6-phosphatase in HepG2 cells.