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The mechanisms underlying false positive results of cfDNA-based non-invasive prenatal screening remain incompletely elucidated.7 Explanatory hypotheses include maternal mosaicism,8,9 undetected tumors,10 the vanishing twin syndrome,11 and confined placental mosaicism,12,13 in addition to technical errors. Although case reports have documented examples of underlying factors behind false positive and various other aberrant results, only a small proportion have already been comprehensively explained.8 Methods of cfDNA-based non-invasive prenatal screening include massively multiplex polymerase-chain-reaction assay,14 shotgun sequencing,15,16 and targeted sequencing.17 The Illumina Verifi and Sequenom MaterniT21 PLUS tests derive from counting statistics that naturally arise from shotgun sequencing of total cfDNA in maternal plasma.